I think I used it 2 x a day for 4 days. I think I felt relief after the first day, but not totally. We tried diflucan and nystatin which did nothing. They were expensive, and the GV was less than $2! I also took acidophilus - it's the active ingredient in yogurt, I washed everything that came in contact with my breasts or DS's mouth in hot water, and limited sweets and startchy foods for a while. I even used paper towels to dry my hands so that I didn't infect the towels. And I only used a bath towel once before washing it in hot water. We did a lot of laundry for a while.
Good luck! I hope you're feeling better today.