STAY FAR AWAY FROM BIOSPOT!!!! It has caused a TON of problems in dogs - seizures, etc.
I use Frontline Top Spot on my dogs and Frontline Plus on fosters who have active fleas (the Plus has an additional ingredient that kills larvae which of course my dogs don't need since they don't have fleas). IMO it is one of the safest spot ons, but it is still chemicals and kids should not pet the dog until the treatment has dried. I usually put it on at night so that it is totally dry by the time we get up in the morning. Some people find that they can go 2-3 months between applications for flea prevention, just a month for ticks though.
If you want to steer clear of chemical flea preventative I've heard great things about Miracle Coat No Fly Zone spray and Natures Guardian. They are "natural" products, but the plant derived active components may cause allergic reactions in some pets (and people).
A healthy diet can also go a long way toward keeping your pets flea/tick free - what are you currently feeding?
You generally *must* use a separate heartworm prevention, regardless of what spot on flea/tick treatment you use (except Revolution I think) - I prefer Interceptor since it also catches all worms except tape worms and my holistic vet says it's gentler on their system.
I use a limited vaccine protocol based off the AAHA recommendations. Here's a link to the pdf: http://www.aahanet.org/PublicDocumen...s06Revised.pdf
I do caution you on doing vaccines at home - parvo and distemper are still very real risks for young pups and if vaccines aren't handled correctly you risk them not being effective. I worked at a shelter for a number of years and we saw a number of dogs vax'd at home who got parvo because they were unprotected.
It's not a risk I would take with a puppy.
HTH - let me know if you need more info on any of the above.