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|In the 1930s and 40s, live female Xenopus frogs were used widely in Europe, Australasia and north America in pregnancy testing.
More than 1,800 amphibian species are now judged to be at risk of extinction
A sample of the woman's urine was injected under the frog's skin; if the woman was pregnant, a hormone in her urine caused the frog to ovulate.
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