It actually probably won't shrink, but you never know especially if it was just regular Joanns microfleece. I'm a little anal so I'd probably wash it, you probably won't need to though.
I don't prewash my microfleece or my PUL. It won't shrink and I wash my diapers to seal holes before they go on baby so that gets the sizing out. I like to work with nice flat, non-static cling microfleece so just skip the wash before the sewing on that one. I've never had a problem.
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